Cellobiose dehydrogenase, an active agent in cellulose depolymerization

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Cellobiose dehydrogenase, an active agent in cellulose depolymerization.

The ability of cellobiose dehydrogenase purified from Phanerochaete chrysosporium to modify a Douglas fir kraft pulp was assessed. Although the addition of cellobiose dehydrogenase alone had little effect, supplementation with cellobiose and iron resulted in a substantial reduction in the degree of polymerization of the pulp cellulose. When the reaction was monitored over time, a progressive de...

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Structural basis for cellobiose dehydrogenase action during oxidative cellulose degradation

A new paradigm for cellulose depolymerization by fungi focuses on an oxidative mechanism involving cellobiose dehydrogenases (CDH) and copper-dependent lytic polysaccharide monooxygenases (LPMO); however, mechanistic studies have been hampered by the lack of structural information regarding CDH. CDH contains a haem-binding cytochrome (CYT) connected via a flexible linker to a flavin-dependent d...

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Cellobiose Dehydrogenase Production by the Genus Cladosporium

Cellobiose dehydrogenase (CDH EC.1.1.5.1) is an extracellular enzyme that mainly produced by wood-degrading fungi. It oxidizes cellobiose to cellobionolactone using a wide spectrum of electron acceptors. The key roles of CDH in growth, metabolism, and some other important cellular processes such as cellulose degradation in fungi have been noted. Since the demands for finding new sources of CDH ...

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Studies of cellulose binding by cellobiose dehydrogenase and a comparison with cellobiohydrolase 1.

The binding isotherm to cellulose of cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium has been compared with that of cellobiohydrolase 1 (CBH 1) from Trichoderma reesei. CDH binds more strongly but more sparsely to cellulose than does CBH 1. In a classical Scatchard analysis, a better fit to a one-site binding model was obtained for CDH than for CBH 1. The binding of both enzymes...

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cellobiose dehydrogenase production by the genus cladosporium

cellobiose dehydrogenase (cdh ec.1.1.5.1) is an extracellular enzyme that mainly produced by wood-degrading fungi. it oxidizes cellobiose to cellobionolactone using a wide spectrum of electron acceptors. the key roles of cdh in growth, metabolism, and some other important cellular processes such as cellulose degradation in fungi have been noted. since the demands for finding new sources of cdh ...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1997

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.63.10.3804-3809.1997